Application of Whatman FTA Card Method in Oil Palm DNA Extraction and PCR Analysis
نویسندگان
چکیده
منابع مشابه
extraction and characterization of allium irancum plant extract and its application in the green synthesis of silver nano particles and oxidation of thiocarbony1 compounds
سنتز سبز نانوذرات فلزی (nps) درسالهای اخیر توجه بسیارزیادی را به خود جلب کرده است. زیرا این پروتوکل کم هزینه وسازگار با محیط زیست از روش های استاندارد سنتز. در این پایان نامه ما گزارش میکنیم یک روش ساده و سازگار با محیط زیست برای سنتز نانوذرات نقره با استفاده از محلول آبی عصاره گیاه allium iranicum به عنوان یک عامل کاهش دهنده ی طبیعی. نانو ذرات نقره مشخص شد با استفاده از تکنیک های uv-visible، x...
Evaluation and comparison of FTA card and CTAB DNA extraction methods for non-agricultural taxa1
PREMISE OF THE STUDY An efficient, effective DNA extraction method is necessary for comprehensive analysis of plant genomes. This study analyzed the quality of DNA obtained using paper FTA cards prepared directly in the field when compared to the more traditional cetyltrimethylammonium bromide (CTAB)-based extraction methods from silica-dried samples. METHODS DNA was extracted using FTA cards...
متن کاملwhatman paper (fta cards) for storing and transferring leishmania dna for pcr examination
background: diagnosis of cutaneous leishmaniasis (cl) is often made based on clinical manifestation. correct diagnosis and identification of the parasite are crucial for choosing the effective treatment and for epidemiological studies. on the other hand, determination of leishmania species is necessary for designing appropriate control programs. diagnosis by pcr is becoming a 'gold standard'...
متن کاملFTA card utility for PCR detection of Mycobacterium leprae.
The suitability of the FTA® elute card for the collection of slit skin smear (SSS) samples for PCR detection of Mycobacterium leprae was evaluated. A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved using both FTA® elute cards and 70% ethanol tubes. To compare the efficacy of PCR detection of DNA f...
متن کاملRepeated extraction of DNA from FTA cards
Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanog...
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ژورنال
عنوان ژورنال: Scientific Research Journal
سال: 2019
ISSN: 2289-649X,1675-7009
DOI: 10.24191/srj.v16i2.6306